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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBN1-Specific Polyclonal antibody

UBN1-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20363-1-AP

Synonyms

HIRA binding protein, Protein VT4, ubinuclein 1, UBN1, UBN1-Specific, VT, VT4

Validation Data Gallery

Applications:
  • WB analysis of mouse liver using 20363-1-AP

    WB analysis of mouse liver using 20363-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20363-1-AP (UBN1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 20363-1-AP

    WB analysis of MCF-7 using 20363-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20363-1-AP (UBN1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 20363-1-AP

    IHC staining of human cervical cancer using 20363-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20363-1-AP (UBN1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cervical cancer using 20363-1-AP

    IHC staining of human cervical cancer using 20363-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20363-1-AP (UBN1-Specific antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse liver tissue, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

20363-1-AP targets UBN1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ubinuclein 1
Calculated molecular weight 121 kDa
Observed molecular weight 120-140 kDa
GenBank accession numberNM_016936
Gene symbol UBN1
Gene ID (NCBI) 29855
RRIDAB_10694818
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBN1, also named as Protein VT4, belongs to the ubinuclein family. UBN1 acts as a novel regulator of senescence. It is involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. UBN1 binds to proliferation-promoting genes. It may be required for replication-independent chromatin assembly. The antibody is specific to UBN1.

Protocols

Product Specific Protocols
WB protocol for UBN1-Specific antibody 20363-1-APDownload protocol
IHC protocol for UBN1-Specific antibody 20363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Stem Cell

PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.

Authors - Zhexin Zhu
View Article