Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HeLa using 14415-1-AP (same clone as 14415-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 14415-1-AP (same clone as 14415-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 14415-1-AP (same clone as 14415-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 14415-1-AP (same clone as 14415-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 14415-1-AP (same clone as 14415-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 14415-1-AP (same clone as 14415-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.
WB analysis of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.
WB analysis of rat kidney using 14415-1-AP (same clone as 14415-1-PBS)
rat kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 14415-1-AP (same clone as 14415-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.
IP experiment of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)
IP result of anti-UBE2L3 (IP:14415-1-AP, 3ug; Detection:14415-1-AP 1:2000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

UBE2L3 Polyclonal antibody, PBS Only

UBE2L3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 14415-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14415-1-PBS targets UBE2L3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5758 Product name: Recombinant human UBE2L3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC053368 Sequence: MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD 相同性解析による交差性が予測される生物種
Full Name	ubiquitin-conjugating enzyme E2L 3
Calculated molecular weight	18 kDa
Observed molecular weight	18 kDa
GenBank accession number	BC053368
Gene Symbol	UBE2L3
Gene ID (NCBI)	7332
RRID	AB_2210891
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P68036
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

UBE2L3(Ubiquitin-conjugating enzyme E2 L3) is also named as UBCE7, UBCH7 and belongs to the ubiquitin-conjugating enzyme family. It regulates nuclear hormone receptors transcriptional activity and may play a role in myelopoiesis.UBE2L3 is associated with susceptibility to systemic lupus erythematosus (SLE) and rheumatoid arthritis in European ancestry populations(PMID:22045845).

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サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

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ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 14415-1-AP (same clone as 14415-1-PBS)

WB analysis of NIH/3T3 using 14415-1-AP (same clone as 14415-1-PBS)

WB analysis of mouse testis using 14415-1-AP (same clone as 14415-1-PBS)

WB analysis of HEK-293 using 14415-1-AP (same clone as 14415-1-PBS)

WB analysis of K-562 using 14415-1-AP (same clone as 14415-1-PBS)

WB analysis of A431 using 14415-1-AP (same clone as 14415-1-PBS)

WB analysis of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)

WB analysis of rat kidney using 14415-1-AP (same clone as 14415-1-PBS)

WB analysis of MCF-7 using 14415-1-AP (same clone as 14415-1-PBS)

IP experiment of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)

IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)