Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HeLa using 14415-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 14415-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 14415-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14415-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14415-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 14415-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 14415-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 14415-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14415-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 14415-1-AP
IP result of anti-UBE2L3 (IP:14415-1-AP, 3ug; Detection:14415-1-AP 1:2000) with mouse kidney tissue lysate 4000ug.
IHC staining of human lung cancer using 14415-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14415-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

UBE2L3 Polyclonal antibody

UBE2L3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14415-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

E2 F1, L UBC, UBCE7, UBCH7, UbcM4, UBE2L3, Ubiquitin carrier protein L3, Ubiquitin protein ligase L3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (11)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A431 cells, HEK-293 cells, K-562 cells, MCF-7 cells, mouse kidney tissue, mouse testis tissue, NIH/3T3 cells, rat kidney tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 10 publications below
IHC	See 2 publications below
IP	See 4 publications below

Product Information

14415-1-AP targets UBE2L3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5758 Product name: Recombinant human UBE2L3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC053368 Sequence: MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD 相同性解析による交差性が予測される生物種
Full Name	ubiquitin-conjugating enzyme E2L 3
Calculated molecular weight	18 kDa
Observed molecular weight	18 kDa
GenBank accession number	BC053368
Gene Symbol	UBE2L3
Gene ID (NCBI)	7332
RRID	AB_2210891
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P68036
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

UBE2L3(Ubiquitin-conjugating enzyme E2 L3) is also named as UBCE7, UBCH7 and belongs to the ubiquitin-conjugating enzyme family. It regulates nuclear hormone receptors transcriptional activity and may play a role in myelopoiesis.UBE2L3 is associated with susceptibility to systemic lupus erythematosus (SLE) and rheumatoid arthritis in European ancestry populations(PMID:22045845).

Protocols

Product Specific Protocols
IHC protocol for UBE2L3 antibody 14415-1-AP	Download protocol
IP protocol for UBE2L3 antibody 14415-1-AP	Download protocol
WB protocol for UBE2L3 antibody 14415-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IHC	Cell Commun Signal Metformin alleviates bone loss in ovariectomized mice through inhibition of autophagy of osteoclast precursors mediated by E2F1 Authors - Xudong Xie View Article
pig	WB	Front Immunol Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms. Authors - Xu Ye View Article
human	WB,IP	Bioengineered E2F transcription factor 1 (E2F1) promotes the transforming growth factor TGF-β1 induced human cardiac fibroblasts differentiation through promoting the transcription of CCNE2 gene Authors - Rongheng Liao KD Validated View Article
pig	WB	Mol Cell Proteomics The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators Authors - Nitchakarn Kaokhum View Article
human	WB	Mediators Inflamm UBC Mediated by SEPT6 Inhibited the Progression of Prostate Cancer. Authors - Ruochen Zhang KD Validated View Article
human,mouse	WB	J Virol The membrane-associated ubiquitin ligase MARCHF8 stabilizes the human papillomavirus oncoprotein E7 by degrading CUL1 and UBE2L3 in head and neck cancer Authors - Mohamed I Khalil View Article

Validation Data Gallery

WB analysis of HeLa using 14415-1-AP

WB analysis of NIH/3T3 using 14415-1-AP

WB analysis of mouse testis using 14415-1-AP

WB analysis of HEK-293 using 14415-1-AP

WB analysis of K-562 using 14415-1-AP

WB analysis of A431 using 14415-1-AP

WB analysis of mouse kidney using 14415-1-AP

WB analysis of rat kidney using 14415-1-AP

WB analysis of MCF-7 using 14415-1-AP

IP experiment of mouse kidney using 14415-1-AP

IHC staining of human lung cancer using 14415-1-AP

IHC staining of human lung cancer using 14415-1-AP

UBE2L3 Polyclonal antibody

UBE2L3 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Metformin alleviates bone loss in ovariectomized mice through inhibition of autophagy of osteoclast precursors mediated by E2F1

Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.

E2F transcription factor 1 (E2F1) promotes the transforming growth factor TGF-β1 induced human cardiac fibroblasts differentiation through promoting the transcription of CCNE2 gene

The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators

UBC Mediated by SEPT6 Inhibited the Progression of Prostate Cancer.

The membrane-associated ubiquitin ligase MARCHF8 stabilizes the human papillomavirus oncoprotein E7 by degrading CUL1 and UBE2L3 in head and neck cancer

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