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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated UBE1 Monoclonal antibody

UBE1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H7E8

Cat no : CL488-67198

Synonyms

A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1

Validation Data Gallery

Applications:
  • WB analysis using CL488-67198

    WB analysis using CL488-67198

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67198 (UBE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy UBA1 Ready-To-Use IHC Kit. UBA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67198-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

CL488-67198 targets UBE1 in WB applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen UBE1 fusion protein Ag8920 相同性解析による交差性が予測される生物種
Full Name ubiquitin-like modifier activating enzyme 1
Calculated molecular weight 1058 aa, 118 kDa
Observed molecular weight 114-118 kDa
GenBank accession numberBC013041
Gene symbol UBA1
Gene ID (NCBI) 7317
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 UBE1 antibody CL488-67198Download protocol
Standard Protocols
Click here to view our Standard Protocols