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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UACA Polyclonal antibody (25654-1-AP)

    WB analysis using 25654-1-AP (same clone as 25654-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25654-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using UACA Polyclonal antibody (25654-1-AP)

    WB analysis of Y79 using 25654-1-AP (same clone as 25654-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25654-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using UACA Polyclonal antibody (25654-1-AP)

    WB analysis of Neuro-2a using 25654-1-AP (same clone as 25654-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25654-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using UACA Polyclonal antibody (25654-1-AP)

    WB analysis of HEK-293 using 25654-1-AP (same clone as 25654-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25654-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using UACA Polyclonal antibody (25654-1-AP)

    IHC staining of human colon using 25654-1-AP (same clone as 25654-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25654-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using UACA Polyclonal antibody (25654-1-AP)

    IHC staining of human colon using 25654-1-AP (same clone as 25654-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25654-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using UACA Polyclonal antibody (25654-1-AP)

    IHC staining of human kidney using 25654-1-AP (same clone as 25654-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25654-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using UACA Polyclonal antibody (25654-1-AP)

    IHC staining of human kidney using 25654-1-AP (same clone as 25654-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25654-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using UACA Polyclonal antibody (25654-1-AP)

    IF Staining of A375 using 25654-1-AP (same clone as 25654-1-PBS)

    Immunofluorescent analysis of A375 cells using 25654-1-AP (UACA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25654-1-PBS in a different storage buffer formulation.

UACA Polyclonal antibody, PBS Only

UACA Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25654-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA1561, NUCLING, Uveal autoantigen with coiled-coil domains and ankyrin repeats

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25654-1-PBS targets UACA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22438

Product name: Recombinant human UACA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1075-1416 aa of BC113407

Sequence: LSQKYTEVKNVKEKLVEENAKQTSEILAVQNLLQKQHVPLEQVEALKKSLNGTIENLKEELKSMQRCYEKEQQTVTKLHQLLENQKNSSVPLAEHLQIKEAFEKEVGIIKASLREKEEESQNKMEEVSKLQSEVQNTKQALKKLETREVVDLSKYKATKSDLETQISSLNEKLANLNRKYEEVCEEVLHAKKKEISAKDEKELLHFSIEQEIKDQKERCDKSLTTITELQRRIQESAKQIEAKDNKITELLNDVERLKQALNGLSQLTYTSGNPTKRQSQLIDTLQHQVKSLEQQLADADRQHQEVIAIYRTHLLSAAQGHMDEDVQEALLQIIQMRQGLVC

相同性解析による交差性が予測される生物種
Full Name uveal autoantigen with coiled-coil domains and ankyrin repeats
Calculated molecular weight 1416 aa, 163 kDa
Observed molecular weight 163 kDa
GenBank accession numberBC113407
Gene Symbol UACA
Gene ID (NCBI) 55075
RRIDAB_2880179
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZF9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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