Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 25654-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of Neuro-2a using 25654-1-AP
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 25654-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 25654-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 25654-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon using 25654-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 25654-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 25654-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 40x lens).
IF Staining of A375 using 25654-1-AP
Immunofluorescent analysis of A375 cells using 25654-1-AP (UACA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

UACA Polyclonal antibody

UACA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25654-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA1561, NUCLING, Uveal autoantigen with coiled-coil domains and ankyrin repeats

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A375 cells, HEK-293 cells, Neuro-2a cells, Y79 cells, HeLa cells
Positive IHC detected in	human colon tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A375 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 2 publications below

Product Information

25654-1-AP targets UACA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22438 Product name: Recombinant human UACA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1075-1416 aa of BC113407 Sequence: LSQKYTEVKNVKEKLVEENAKQTSEILAVQNLLQKQHVPLEQVEALKKSLNGTIENLKEELKSMQRCYEKEQQTVTKLHQLLENQKNSSVPLAEHLQIKEAFEKEVGIIKASLREKEEESQNKMEEVSKLQSEVQNTKQALKKLETREVVDLSKYKATKSDLETQISSLNEKLANLNRKYEEVCEEVLHAKKKEISAKDEKELLHFSIEQEIKDQKERCDKSLTTITELQRRIQESAKQIEAKDNKITELLNDVERLKQALNGLSQLTYTSGNPTKRQSQLIDTLQHQVKSLEQQLADADRQHQEVIAIYRTHLLSAAQGHMDEDVQEALLQIIQMRQGLVC 相同性解析による交差性が予測される生物種
Full Name	uveal autoantigen with coiled-coil domains and ankyrin repeats
Calculated molecular weight	1416 aa, 163 kDa
Observed molecular weight	163 kDa
GenBank accession number	BC113407
Gene Symbol	UACA
Gene ID (NCBI)	55075
RRID	AB_2880179
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BZF9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for UACA antibody 25654-1-AP	Download protocol
IHC protocol for UACA antibody 25654-1-AP	Download protocol
WB protocol for UACA antibody 25654-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Aging (Albany NY) Transcriptome and proteome analysis of the antitumor activity of maslinic acid against pancreatic cancer cells Authors - Hewei Zhang View Article
mouse	IF	J Zhejiang Univ Sci B Roles of PANoptosis and related genes in acute liver failure: neoteric insight from bioinformatics analysis and animal experiment verification Authors - Tiantian Ge View Article
human	WB	J Oncol Maslinic Acid Inhibits the Growth of Malignant Gliomas by Inducing Apoptosis via MAPK Signaling. Authors - Yongqiang Wang View Article
canine	WB	Cell Rep Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells. Authors - Takahide Matsui View Article
human	WB,IF	Int J Clin Exp Pathol Knockdown of UACA inhibitsproliferation and invasion and promotes senescence of hepatocellular carcinoma cells Authors - Jing-Yuan Sun KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 25654-1-AP

WB analysis of Neuro-2a using 25654-1-AP

WB analysis of HEK-293 using 25654-1-AP

WB analysis of Y79 using 25654-1-AP

IHC staining of human colon using 25654-1-AP

IHC staining of human colon using 25654-1-AP

IHC staining of human kidney using 25654-1-AP

IHC staining of human kidney using 25654-1-AP

IF Staining of A375 using 25654-1-AP

UACA Polyclonal antibody

UACA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Transcriptome and proteome analysis of the antitumor activity of maslinic acid against pancreatic cancer cells

Roles of PANoptosis and related genes in acute liver failure: neoteric insight from bioinformatics analysis and animal experiment verification

Maslinic Acid Inhibits the Growth of Malignant Gliomas by Inducing Apoptosis via MAPK Signaling.

Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.

Knockdown of UACA inhibitsproliferation and invasion and promotes senescence of hepatocellular carcinoma cells