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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated TrpRS Monoclonal antibody

TrpRS Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C6C3

Cat no : CL488-67952

Synonyms

GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS

Validation Data Gallery

Applications:
  • IF Staining of MCF-7 using CL488-67952

    IF Staining of MCF-7 using CL488-67952

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 TrpRS antibody (CL488-67952, Clone: 1C6C3 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67952

    FC experiment of HeLa using CL488-67952

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TrpRS (CL488-67952, Clone:1C6C3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inMCF-7 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy WARS Ready-To-Use IHC Kit. WARS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67952-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67952 targets TrpRS in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TrpRS fusion protein Ag31430 相同性解析による交差性が予測される生物種
Full Name tryptophanyl-tRNA synthetase
Calculated molecular weight 471 aa, 53 kDa
Observed molecular weight53 kDa
GenBank accession numberBC017489
Gene symbol WARS
Gene ID (NCBI) 7453
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TrpRS antibody CL488-67952Download protocol
FC protocol for CL Plus 488 TrpRS antibody CL488-67952Download protocol
Standard Protocols
Click here to view our Standard Protocols