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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Transgelin 2 Monoclonal antibody

Transgelin 2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E5G1

Cat no : CL488-60044

Synonyms

HA1756, KIAA0120, SM22 alpha homolog, SM22 beta, SM22beta, TAGL2, TAGLN2, Transgelin 2

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-60044

    IF Staining of HepG2 using CL488-60044

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Transgelin 2 antibody (CL488-60044, Clone: 2E5G1 ) at dilution of 1:200.

  • IF Staining of human colon cancer using CL488-60044

    IF Staining of human colon cancer using CL488-60044

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 Transgelin 2 antibody (CL488-60044, Clone: 2E5G1 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells, human colon cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 60044-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-60044 targets Transgelin 2 in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Transgelin 2 fusion protein Ag0337 相同性解析による交差性が予測される生物種
Full Name transgelin 2
Calculated molecular weight 199 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC002616
Gene symbol TAGLN2
Gene ID (NCBI) 8407
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. Our test result showed that this antibody Is specific to TAGLN2. It does not bind TAGLN1 and TAGLN3.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Transgelin 2 antibody CL488-60044Download protocol
Standard Protocols
Click here to view our Standard Protocols