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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Tissue Factor/CD142 Monoclonal antibody (67056-1-Ig)

    WB analysis of A431 using 67056-1-Ig (same clone as 67056-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 67056-1-Ig (Tissue Factor antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human blood using Tissue Factor/CD142 Monoclonal antibody (67056-1-Ig)

    WB analysis of human blood using 67056-1-Ig (same clone as 67056-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 67056-1-Ig (Tissue Factor antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67056-1-PBS in a different storage buffer formulation.

Tissue Factor/CD142 Monoclonal antibody, PBS Only

Tissue Factor/CD142 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67056-1-PBS
Clone No.2E4A1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

CD142, Coagulation factor III, F3, Tissue factor, 2E4A1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67056-1-PBS targets Tissue Factor/CD142 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27563

Product name: Recombinant human F3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 113-251 aa of BC011029

Sequence: GNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFRE

相同性解析による交差性が予測される生物種
Full Name coagulation factor III (thromboplastin, tissue factor)
Calculated molecular weight 295 aa, 33 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC011029
Gene Symbol F3
Gene ID (NCBI) 2152
RRIDAB_2882367
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP13726
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.