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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Tissue Factor Monoclonal antibody

Tissue factor Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2E4A1

Cat no : 67056-1-Ig

Synonyms

CD142, Coagulation factor III, F3, TF, TFA, Thromboplastin, Tissue factor

Validation Data Gallery

Applications:
  • WB analysis of A431 using 67056-1-Ig

    WB analysis of A431 using 67056-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67056-1-Ig (Tissue factor antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of human blood using 67056-1-Ig

    WB analysis of human blood using 67056-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 67056-1-Ig (Tissue Factor antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HUVEC using 67056-1-Ig

    FC experiment of HUVEC using 67056-1-Ig

    1X10^6 HUVEC cells were intracellularly stained with 0.2 ug Anti-Human Tissue Factor (67056-1-Ig, Clone:2E4A1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA431 cells, human blood tissue
Positive FC detected inHUVEC cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67056-1-Ig targets Tissue factor in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Tissue factor fusion protein Ag27563 相同性解析による交差性が予測される生物種
Full Name coagulation factor III (thromboplastin, tissue factor)
Calculated molecular weight 295 aa, 33 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC011029
Gene symbol F3
Gene ID (NCBI) 2152
RRIDAB_2882367
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Tissue factor antibody 67056-1-IgDownload protocol
FC protocol for Tissue factor antibody 67056-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols