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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Talin-1 Recombinant monoclonal antibody (82856-4-RR)

    WB analysis using 82856-4-RR (same clone as 82856-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82856-4-RR (Talin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Talin-1 Recombinant monoclonal antibody (82856-4-RR)

    IP experiment of HeLa using 82856-4-RR (same clone as 82856-4-PBS)

    IP result of anti-Talin-1 (IP:82856-4-RR, 4ug; Detection:82856-4-RR 1:3000) with HeLa cells lysate 1120 ug. This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Talin-1 Recombinant monoclonal antibody (82856-4-RR)

    IHC staining of mouse colon using 82856-4-RR (same clone as 82856-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Talin-1 Recombinant monoclonal antibody (82856-4-RR)

    IHC staining of mouse colon using 82856-4-RR (same clone as 82856-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Talin-1 Recombinant monoclonal antibody (82856-4-RR)

    IHC staining of human colon using 82856-4-RR (same clone as 82856-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Talin-1 Recombinant monoclonal antibody (82856-4-RR)

    IHC staining of human colon using 82856-4-RR (same clone as 82856-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Recombinant monoclonal antibody (82856-4-RR)

    IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Talin-1 Recombinant monoclonal antibody (82856-4-RR)

    IF Staining of HUVEC using 82856-4-RR (same clone as 82856-4-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Recombinant monoclonal antibody (82856-4-RR)

    IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Recombinant monoclonal antibody (82856-4-RR)

    IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Recombinant monoclonal antibody (82856-4-RR)

    IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82856-4-RR

    Affinity and Kinetic Characterization of 82856-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82856-4-RR against Human TLN1 were performed. The affinity constant is 0.215 nM.

Talin-1 Recombinant monoclonal antibody, PBS Only

Talin-1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82856-4-PBS
Clone No.7D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

7D11, ILWEQ, KIAA1027, talin 1, talin1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82856-4-PBS targets Talin-1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5367

Product name: Recombinant human TLN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2193-2541 aa of BC042923

Sequence: GNSCRQEDVIATANLSRRAIADMLRACKEAAYHPEVAPDVRLRALHYGRECANGYLELLDHVLLTLQKPSPELKQQLTGHSKRVAGSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANALDDGQWSQGLISAARMVAAATNNLCEAANAAVQGHASQEKLISSAKQVAASTAQLLVACKVKADQDSEAMKRLQAAGNAVKRASDNLVKAAQKAAAFEEQENETVVVKEKMVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELRDEH

相同性解析による交差性が予測される生物種
Full Name talin 1
Calculated molecular weight 2541 aa, 270 kDa
Observed molecular weight270 kDa
GenBank accession numberBC042923
Gene Symbol Talin-1
Gene ID (NCBI) 7094
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y490
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

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