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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TXNRD3 Polyclonal antibody (19517-1-AP)

    WB analysis using 19517-1-AP (same clone as 19517-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19517-1-AP (TXNRD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19517-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using TXNRD3 Polyclonal antibody (19517-1-AP)

    WB analysis of human testis using 19517-1-AP (same clone as 19517-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 19517-1-AP (TXNRD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19517-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TXNRD3 Polyclonal antibody (19517-1-AP)

    IHC staining of mouse testis using 19517-1-AP (same clone as 19517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19517-1-AP (TXNRD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19517-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TXNRD3 Polyclonal antibody (19517-1-AP)

    IHC staining of mouse testis using 19517-1-AP (same clone as 19517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19517-1-AP (TXNRD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19517-1-PBS in a different storage buffer formulation.

TXNRD3 Polyclonal antibody, PBS Only

TXNRD3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 19517-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19517-1-PBS targets TXNRD3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name thioredoxin reductase 3
Calculated molecular weight 75 kDa
Observed molecular weight 82 kDa
GenBank accession numberXM_001130163
Gene Symbol TXNRD3
Gene ID (NCBI) 114112
RRIDAB_10644343
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VQ6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TXNRD3, also named as TGR and TRXR3, belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. TXNRD3 displays thioredoxin reductase, glutaredoxin and glutathione reductase activities. TXNRD3 catalyzes the reaction: Thioredoxin + NADP+ = thioredoxin disulfide + NADPH. TXNRD3 promotes disulfide bond formation between GPX4 and various sperm proteins and may play a role in sperm maturation by promoting formation of sperm structural components.

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