Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, human testis tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
19517-1-AP targets TXNRD3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | thioredoxin reductase 3 |
| Calculated molecular weight | 75 kDa |
| Observed molecular weight | 82 kDa |
| GenBank accession number | XM_001130163 |
| Gene Symbol | TXNRD3 |
| Gene ID (NCBI) | 114112 |
| RRID | AB_10644343 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VQ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TXNRD3, also named as TGR and TRXR3, belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. TXNRD3 displays thioredoxin reductase, glutaredoxin and glutathione reductase activities. TXNRD3 catalyzes the reaction: Thioredoxin + NADP+ = thioredoxin disulfide + NADPH. TXNRD3 promotes disulfide bond formation between GPX4 and various sperm proteins and may play a role in sperm maturation by promoting formation of sperm structural components.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TXNRD3 antibody 19517-1-AP | Download protocol |
| WB protocol for TXNRD3 antibody 19517-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer | ||
Nat Chem Biol Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability. | ||
Front Vet Sci The Selenium Yeast vs Selenium Methionine on Cell Viability, Selenoprotein Profile and Redox Status via JNK/ P38 Pathway in Porcine Mammary Epithelial Cells. | ||
Life Sci Supression Thioredoxin reductase 3 exacerbates the progression of liver cirrhosis via activation of ferroptosis pathway |
