Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-84756-5 targets TXNDC5 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag13965 Product name: Recombinant human TXNDC5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-324 aa of BC001199 Sequence: MEDAKVYVAKVDCTAHSDVCSAQGVRGYPTLKLFKPGQEAVKYQGPRDFQTLENWMLQTLNEEPVTPEPEVEPPSAPELKQGLYELSASNFELHVAQGDHFIKFFAPWCGHCKALAPTWEQLALGLEHSETVKIGKVDCTQHYELCSGNQVRGYPTLLWFRDGKKVDQYKGKRDLESLREYVESQLQRTETGATETVTPSEAPVLAAEPEADKGTVLALTENNFDDTIAEGITFIKFYAPWCGHCKTLAPTWEELSKKEFPGLAGVKIAEVDCTAERNICSKYSVRGYPTLLLFRGGKKVSEHSGGRDLDSLHRFVLSQAKDEL 相同性解析による交差性が予測される生物種 |
| Full Name | thioredoxin domain containing 5 (endoplasmic reticulum) |
| Calculated molecular weight | 432 aa, 48 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC001199 |
| Gene Symbol | TXNDC5 |
| Gene ID (NCBI) | 81567 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NBS9 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TXNDC5, or Thioredoxin Domain Containing 5, is a protein-coding gene that plays a significant role in cellular processes, particularly in the endoplasmic reticulum (ER). This gene is essential for the proper functioning of the cellular environment, primarily through its involvement in the formation and rearrangement of disulfide bonds within proteins. Disulfide bonds are crucial for the stability and functionality of many proteins, and TXNDC5 facilitates these processes through its thioredoxin domains, which contain enzymatic activities that promote correct protein folding and maturation in the ER. Recent studies highlight the expression characteristics of TXNDC5 across various diseases. It has been implicated in the promotion of malignant diseases, suggesting a potential role in cancer biology and other pathological conditions. (PMID:25526565; 38629066)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TXNDC5 antibody CL488-84756-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
