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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis using 20811-1-AP (same clone as 20811-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of Jurkat using 20811-1-AP (same clone as 20811-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of HEK-293 using 20811-1-AP (same clone as 20811-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of HeLa using 20811-1-AP (same clone as 20811-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of K-562 using 20811-1-AP (same clone as 20811-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of human kidney using 20811-1-AP (same clone as 20811-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of mouse pancreas using 20811-1-AP (same clone as 20811-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human uterus tissue using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of human uterus using 20811-1-AP (same clone as 20811-1-PBS)

    human uterus tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of L02 using 20811-1-AP (same clone as 20811-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    IP experiment of HEK-293 using 20811-1-AP (same clone as 20811-1-PBS)

    IP result of anti-TXNDC17 (IP:20811-1-AP, 4ug; Detection:20811-1-AP 1:6000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    IF Staining of HepG2 using 20811-1-AP (same clone as 20811-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TXNDC17 antibody (20811-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    IF Staining of HeLa using 20811-1-AP (same clone as 20811-1-PBS)

    Immunofluorescent analysis of Hela cells, using TXNDC17 antibody 20811-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    IF Staining of HepG2 using 20811-1-AP (same clone as 20811-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using TXNDC17 antibody 20811-1-AP at 1:25 dilution and FITC- labeled donkey anti-Rabbit IgG (green). This data was developed using the same antibody clone with 20811-1-PBS in a different storage buffer formulation.

TXNDC17 Polyclonal antibody, PBS Only

TXNDC17 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 20811-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

TXNL5, TRP14, Thioredoxin-like protein 5, Thioredoxin domain-containing protein 17, Protein 42-9-9

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20811-1-PBS targets TXNDC17 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14766

Product name: Recombinant human TXNDC17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-123 aa of BC006405

Sequence: MARYEEVSVSGFEEFHRAVEQHNGKTIFAYFTGSKDAGGKSWCPDCVQAEPVVREGLKHISEGCVFIYCQVGEKPYWKDPNNDFRKNLKVTAVPTLLKYGTPQKLVESECLQANLVEMLFSED

相同性解析による交差性が予測される生物種
Full Name thioredoxin domain containing 17
Calculated molecular weight 123 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC006405
Gene Symbol TXNDC17
Gene ID (NCBI) 84817
RRIDAB_10695499
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRA2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TXNDC17 also known as TRP14, TXNL5, belongs to the thioredoxin family. TRP14 is a widely expressed, 123-amino acid protein with a WCPDC motif. TXNDC17 has a thioredoxin fold and a TRX-like active-site sequence(PMID: 14607844, 24778250).

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