Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis using 20811-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 20811-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20811-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20811-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 20811-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 20811-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 20811-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 20811-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human uterus using 20811-1-AP
human uterus tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 20811-1-AP
IP result of anti-TXNDC17 (IP:20811-1-AP, 4ug; Detection:20811-1-AP 1:6000) with HEK-293 cells lysate 1575 ug.
IF Staining of HepG2 using 20811-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TXNDC17 antibody (20811-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HeLa using 20811-1-AP
Immunofluorescent analysis of Hela cells, using TXNDC17 antibody 20811-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HepG2 using 20811-1-AP
Immunofluorescent analysis of HepG2 cells, using TXNDC17 antibody 20811-1-AP at 1:25 dilution and FITC- labeled donkey anti-Rabbit IgG (green).

TXNDC17 Polyclonal antibody

TXNDC17 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 20811-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

TXNL5, TRP14, Thioredoxin-like protein 5, Thioredoxin domain-containing protein 17, Protein 42-9-9

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HEK-293 cells, HeLa cells, human kidney tissue, human uterus tissue, Jurkat cells, K-562 cells, L02 cells, mouse pancreas tissue
Positive IP detected in	HEK-293 cells
Positive IF/ICC detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

20811-1-AP targets TXNDC17 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14766 Product name: Recombinant human TXNDC17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-123 aa of BC006405 Sequence: MARYEEVSVSGFEEFHRAVEQHNGKTIFAYFTGSKDAGGKSWCPDCVQAEPVVREGLKHISEGCVFIYCQVGEKPYWKDPNNDFRKNLKVTAVPTLLKYGTPQKLVESECLQANLVEMLFSED 相同性解析による交差性が予測される生物種
Full Name	thioredoxin domain containing 17
Calculated molecular weight	123 aa, 14 kDa
Observed molecular weight	14 kDa
GenBank accession number	BC006405
Gene Symbol	TXNDC17
Gene ID (NCBI)	84817
RRID	AB_10695499
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BRA2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TXNDC17 also known as TRP14, TXNL5, belongs to the thioredoxin family. TRP14 is a widely expressed, 123-amino acid protein with a WCPDC motif. TXNDC17 has a thioredoxin fold and a TRX-like active-site sequence(PMID: 14607844, 24778250).

Protocols

Product Specific Protocols
IF protocol for TXNDC17 antibody 20811-1-AP	Download protocol
IP protocol for TXNDC17 antibody 20811-1-AP	Download protocol
WB protocol for TXNDC17 antibody 20811-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Int J Radiat Biol Combination of podophyllotoxin and rutin modulate radiation-induced alterations of jejunal proteome in mice. Authors - Sania Bajaj View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 20811-1-AP

WB analysis of Jurkat using 20811-1-AP

WB analysis of HEK-293 using 20811-1-AP

WB analysis of HeLa using 20811-1-AP

WB analysis of K-562 using 20811-1-AP

WB analysis of human kidney using 20811-1-AP

WB analysis of mouse pancreas using 20811-1-AP

WB analysis of L02 using 20811-1-AP

WB analysis of human uterus using 20811-1-AP

IP experiment of HEK-293 using 20811-1-AP

IF Staining of HepG2 using 20811-1-AP

IF Staining of HeLa using 20811-1-AP