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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTC9C Polyclonal antibody (31797-1-AP)

    WB analysis using 31797-1-AP (same clone as 31797-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31797-1-AP (TTC9C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31797-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TTC9C Polyclonal antibody (31797-1-AP)

    WB analysis of HeLa using 31797-1-AP (same clone as 31797-1-PBS)

    WB result of TTC9C antibody (31797-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC9C transfected HeLa cells. This data was developed using the same antibody clone with 31797-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TTC9C Polyclonal antibody (31797-1-AP)

    IHC staining of mouse testis using 31797-1-AP (same clone as 31797-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31797-1-AP (TTC9C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31797-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using TTC9C Polyclonal antibody (31797-1-AP)

    IF Staining of mouse testis using 31797-1-AP (same clone as 31797-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TTC9C antibody (31797-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31797-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using TTC9C Polyclonal antibody (31797-1-AP)

    IF Staining of hTERT-RPE1 using 31797-1-AP (same clone as 31797-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTC9C antibody (31797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 31797-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TTC9C Polyclonal antibody, PBS Only

TTC9C Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 31797-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Tetratricopeptide repeat protein 9C, TPR repeat protein 9C

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31797-1-PBS targets TTC9C in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag37081

Product name: Recombinant human TTC9C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-171 aa of BC032123

Sequence: MEKRLQEAQLYKEEGNQRYREGKYRDAVSRYHRALLQLRGLDPSLPSPLPNLGPQGPALTPEQENILHTTQTDCYNNLAACLLQMEPVNYERVREYSQKVLERQPDNAKALYRAGVAFFHLQDYDQARHYLLAAVNRQPKDANVRRYLQLTQSELSSYHRKEKQLYLGMFG

相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 9C
Observed molecular weight22 kDa
GenBank accession numberBC032123
Gene Symbol TTC9C
Gene ID (NCBI) 283237
RRIDAB_3670117
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8N5M4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TTC9 family belongs to tetratricopeptide repeat (TPR)-containing proteins family (PMID: 35949301).The TPR motifs are arranged in antiparallel α-helical hairpins which are clustered to form flexible grooved interfaces. Members in TRP-containing proteins family are involved in a diverse range of biological functions, including cell cycle control, transcription, protein folding, and steroid receptor signaling (PMID: 15497498;PMID: 10786835).

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