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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTC9C Polyclonal antibody (31797-1-AP)

    WB analysis using 31797-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31797-1-AP (TTC9C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TTC9C Polyclonal antibody (31797-1-AP)

    IHC staining of mouse testis using 31797-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31797-1-AP (TTC9C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using TTC9C Polyclonal antibody (31797-1-AP)

    IF Staining of mouse testis using 31797-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TTC9C antibody (31797-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using TTC9C Polyclonal antibody (31797-1-AP)

    IF Staining of hTERT-RPE1 using 31797-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTC9C antibody (31797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

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TTC9C Polyclonal antibody

TTC9C Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 31797-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

TPR repeat protein 9C, Tetratricopeptide repeat protein 9C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells, HeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31797-1-AP targets TTC9C in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC9C fusion protein Ag37081 相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 9C
Observed molecular weight22 kDa
GenBank accession numberBC032123
Gene Symbol TTC9C
Gene ID (NCBI) 283237
RRIDAB_3670117
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8N5M4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TTC9 family belongs to tetratricopeptide repeat (TPR)-containing proteins family (PMID: 35949301).The TPR motifs are arranged in antiparallel α-helical hairpins which are clustered to form flexible grooved interfaces. Members in TRP-containing proteins family are involved in a diverse range of biological functions, including cell cycle control, transcription, protein folding, and steroid receptor signaling (PMID: 15497498;PMID: 10786835).

Protocols

Product Specific Protocols
WB protocol for TTC9C antibody 31797-1-APDownload protocol
IHC protocol for TTC9C antibody 31797-1-APDownload protocol
IF protocol for TTC9C antibody 31797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols