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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite® Plus 488-conjugated TTC30A Polyclonal an (CL488-25352)

    IF Staining of hTERT-RPE1 using CL488-25352

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 TTC30A antibody (CL488-25352) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TTC30A Polyclonal antibody

TTC30A Polyclonal Antibody for IF/ICC
Cat No. CL488-25352

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

IFT70, TPR repeat protein 30A, TTC30A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-25352 targets TTC30A in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC30A fusion protein Ag18361 相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 30A
Calculated molecular weight 665 aa, 76 kDa
Observed molecular weight 72-76 kDa
GenBank accession numberBC042848
Gene Symbol TTC30A
Gene ID (NCBI) 92104
RRIDAB_3672792
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WT1
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tetratricopeptide repeat protein 30A (TTC30A) as a component of ciliary segmentation, may be essential for cartilage differentiation and renal tubulogenesis (PMID: 34548398). It is required for polyglutamylation of axonemal tubulin and plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TTC30A antibody CL488-25352Download protocol
Standard Protocols
Click here to view our Standard Protocols