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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTC28 Polyclonal antibody (25701-1-AP)

    WB analysis using 25701-1-AP (same clone as 25701-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25701-1-AP (TTC28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TTC28 Polyclonal antibody (25701-1-AP)

    WB analysis of HeLa using 25701-1-AP (same clone as 25701-1-PBS)

    WB result of TTC28 antibody (25701-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC28 transfected HeLa cells. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TTC28 Polyclonal antibody (25701-1-AP)

    WB analysis using 25701-1-AP (same clone as 25701-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25701-1-AP (TTC28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using TTC28 Polyclonal antibody (25701-1-AP)

    IP experiment of HEK-293 using 25701-1-AP (same clone as 25701-1-PBS)

    IP result of anti-TTC28 (IP:25701-1-AP, 4ug; Detection:25701-1-AP 1:2500) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TTC28 Polyclonal antibody, PBS Only

TTC28 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 25701-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

TPR repeat protein 28, TPR repeat-containing big gene cloned at Keio, TPRBK

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25701-1-PBS targets TTC28 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22465

Product name: Recombinant human TTC28 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 906-1247 aa of BC016465

Sequence: TGESDQSSTETDSTVKSQEESNPKLDPQELAQKILEETQSHLIAVERLQRSGGQVSKSNNPEDGVQAPSSTAVFRASETSAFSRPVLSHQKSQPSPVTVKPKPPARSSSLPKVSSGYSSPTTSEMSIKDSPSQHSGRPSPGCDSQTSQLDQPLFKLKYPSSPYSAHISKSPRNMSPSSGHQSPAGSAPSPALSYSSAGSARSSPADAPDIDKLKMAAIDEKVQAVHNLKMFWQSTPQHSTGPMKIFRGAPGTMTSKRDVLSLLNLSPRHNKKEEGVDKLELKELSLQQHDGAPPKAPPNGHWRTETTSLGSLPLPAGPPATAPARPLRLPSGNGYKFLSPGR

相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 28
Calculated molecular weight 2481 aa, 271 kDa
Observed molecular weight240-271 kDa
GenBank accession numberBC016465
Gene Symbol TTC28
Gene ID (NCBI) 23331
RRIDAB_3085817
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AY4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TTC28 is a large protein required during the cell cycle for condensation of spindle midzone microtubules, formation of the midbody, and completion of cytokinesis. RT-PCR ELISA detected highest TTC28 expression in adult ovary and lung, followed by kidney and liver. Lower expression was detected in all other adult and fetal tissues examined except testis, which showed no expression.

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