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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTC28 Polyclonal antibody (25701-1-AP)

    WB analysis using 25701-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25701-1-AP (TTC28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TTC28 Polyclonal antibody (25701-1-AP)

    WB analysis of HeLa using 25701-1-AP

    WB result of TTC28 antibody (25701-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC28 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using TTC28 Polyclonal antibody (25701-1-AP)

    IP experiment of HEK-293 using 25701-1-AP

    IP result of anti-TTC28 (IP:25701-1-AP, 4ug; Detection:25701-1-AP 1:2500) with HEK-293 cells lysate 1480 ug.

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TTC28 Polyclonal antibody

TTC28 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25701-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

KIAA1043, TPR repeat protein 28, TTC28

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25701-1-AP targets TTC28 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC28 fusion protein Ag22465 相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 28
Calculated molecular weight 2481 aa, 271 kDa
Observed molecular weight240-271 kDa
GenBank accession numberBC016465
Gene Symbol TTC28
Gene ID (NCBI) 23331
RRIDAB_3085817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TTC28 is a large protein required during the cell cycle for condensation of spindle midzone microtubules, formation of the midbody, and completion of cytokinesis. RT-PCR ELISA detected highest TTC28 expression in adult ovary and lung, followed by kidney and liver. Lower expression was detected in all other adult and fetal tissues examined except testis, which showed no expression.

Protocols

Product Specific Protocols
WB protocol for TTC28 antibody 25701-1-APDownload protocol
IP protocol for TTC28 antibody 25701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols