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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TSPAN31 Polyclonal antibody (21987-1-AP)

    WB analysis of HeLa using 21987-1-AP (same clone as 21987-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21987-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TSPAN31 Polyclonal antibody (21987-1-AP)

    WB analysis using 21987-1-AP (same clone as 21987-1-PBS)

    Rat heart tissue was subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21987-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TSPAN31 Polyclonal antibody (21987-1-AP)

    WB analysis of HepG2 using 21987-1-AP (same clone as 21987-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21987-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using TSPAN31 Polyclonal antibody (21987-1-AP)

    WB analysis of PC-3 using 21987-1-AP (same clone as 21987-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21987-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using TSPAN31 Polyclonal antibody (21987-1-AP)

    WB analysis of mouse skeletal muscle using 21987-1-AP (same clone as 21987-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21987-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TSPAN31 Polyclonal antibody (21987-1-AP)

    IHC staining of human skeletal muscle using 21987-1-AP (same clone as 21987-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21987-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TSPAN31 Polyclonal antibody (21987-1-AP)

    IHC staining of human skeletal muscle using 21987-1-AP (same clone as 21987-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21987-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TSPAN31 Polyclonal antibody (21987-1-AP)

    IHC staining of human heart using 21987-1-AP (same clone as 21987-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21987-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TSPAN31 Polyclonal antibody (21987-1-AP)

    IHC staining of human heart using 21987-1-AP (same clone as 21987-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21987-1-PBS in a different storage buffer formulation.

TSPAN31 Polyclonal antibody, PBS Only

TSPAN31 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21987-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Sarcoma-amplified sequence, SAS, Tetraspanin-31, Tspan-31

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21987-1-PBS targets TSPAN31 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17138

Product name: Recombinant human TSPAN31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 91-180 aa of BC010377

Sequence: VISCSCLAINRSKQTDVINASWWVMSNKTRDELERSFDCCGLFNLTTLYQQDYDFCTAICKSQSPTCQMCGEKFLKHSDEALKILGGVGL

相同性解析による交差性が予測される生物種
Full Name tetraspanin 31
Calculated molecular weight 210 aa, 23 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC010377
Gene Symbol TSPAN31
Gene ID (NCBI) 6302
RRIDAB_2878962
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12999
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Tetraspanin-31 (TSPAN31) belongs to the tetraspanin (TM4SF) family, also known as the transmembrane 4 (TM4) superfamily, which is a family of transmembrane proteins with 33 mammalian members. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. Tetraspanin-31 is thought to be involved in growth-related cellular processes. The experimentally determined molecular mass of 30 kDa is larger than the calculated molecular mass of 23 kDa, which could be a result of post-translational modifications.

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