Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 21987-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21987-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 21987-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 21987-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human skeletal muscle using 21987-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skeletal muscle using 21987-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 21987-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 21987-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 40x lens).

TSPAN31 Polyclonal antibody

TSPAN31 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21987-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Sarcoma amplified sequence, SAS, tetraspanin 31, Tspan 31, TSPAN31

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, mouse skeletal muscle tissue, PC-3 cells
Positive IHC detected in	human skeletal muscle tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 2 publications below

Product Information

21987-1-AP targets TSPAN31 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17138 Product name: Recombinant human TSPAN31 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 91-180 aa of BC010377 Sequence: VISCSCLAINRSKQTDVINASWWVMSNKTRDELERSFDCCGLFNLTTLYQQDYDFCTAICKSQSPTCQMCGEKFLKHSDEALKILGGVGL 相同性解析による交差性が予測される生物種
Full Name	tetraspanin 31
Calculated molecular weight	210 aa, 23 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC010377
Gene Symbol	TSPAN31
Gene ID (NCBI)	6302
RRID	AB_2878962
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q12999
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Tetraspanin-31 (TSPAN31) belongs to the tetraspanin (TM4SF) family, also known as the transmembrane 4 (TM4) superfamily, which are a family of transmembrane proteins with 33 mammalian members. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. Tetraspanin-31 is thought to be involved in growth-related cellular processes. The experimentally determined molecular mass of 30 kDa is larger than the calculated molecular mass of 23 kDa, which could be a result of post-translational modifications.

Protocols

Product Specific Protocols
IHC protocol for TSPAN31 antibody 21987-1-AP	Download protocol
WB protocol for TSPAN31 antibody 21987-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Cancer Sci Overexpression of Tetraspanin31 contributes to malignant potential and poor outcomes in gastric cancer Authors - Yusuke Takashima View Article
human	WB,IHC	Mol Carcinog TSPAN31 Activates Fatty Acid Metabolism and PI3K/AKT Pathway to Promote Tumor Progression in Breast Cancer Authors - Wenquan Luo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 21987-1-AP

WB analysis of HepG2 using 21987-1-AP

WB analysis of PC-3 using 21987-1-AP

WB analysis of mouse skeletal muscle using 21987-1-AP

IHC staining of human skeletal muscle using 21987-1-AP