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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Raji cells, L02 cells, C2C12 cells, COS-7 cells, Vero cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85212-2-RR targets TRX2/TXN2 in WB, ELISA applications and shows reactivity with human, mouse, monkey samples.
| Tested Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | TRX2/TXN2 fusion protein Ag3640 相同性解析による交差性が予測される生物種 |
| Full Name | thioredoxin 2 |
| Calculated molecular weight | 166 aa, 18 kDa |
| Observed molecular weight | 12 kDa |
| GenBank accession number | BC013726 |
| Gene Symbol | Thioredoxin 2 |
| Gene ID (NCBI) | 25828 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q99757 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TXN2 (Thioredoxin-2) is also named as TRX2, MTRX, Mt-TRX, and regulates the mitochondrial redox state and plays an important role in cell proliferation. It has been shown that cells conditionally deficient in Trx2 undergo apoptosis in the absence of exogenous stress, accompanied by an accumulation of intracellular ROS, the activation of caspase 9 and caspase 3, and the release of cytochrome c into the cytosol. (PMID: 18441302).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRX2/TXN2 antibody 85212-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |