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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRX2/TXN2 Recombinant antibody (85212-2-RR)

    WB analysis using 85212-2-RR (same clone as 85212-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85212-2-RR (TRX2/TXN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85212-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01917-1

    Sandwich ELISA standard curve of MP01917-1

    Sandwich ELISA standard curve of MP01917-1, Human TRX2/TXN2 Recombinant Matched Antibody Pair - PBS only. 85212-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3640. 85212-1-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL.

  • Sample test of MP01917-1

    Sample test of MP01917-1

    The mean TRX2/TXN2 concentration was determined to be 1,925.24 ng/mL in Raji cell extract based on a 3.00 mg/mL extract load.

  • Cytometric bead array standard curve of MP01917-1

    Cytometric bead array standard curve of MP01917-1

    Cytometric bead array standard curve of MP01917-1, TRX2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85212-2-PBS. Detection antibody: 85212-1-PBS. Standard: Ag3640. Range: 0.781-100 ng/mL.

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TRX2/TXN2 Recombinant antibody, PBS Only (Capture)

TRX2/TXN2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85212-2-PBS
Clone No.242707H4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

TRX2, MT TRX, MTRX, Mt-Trx, Thioredoxin 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85212-2-PBS targets TRX2/TXN2 as part of a matched antibody pair:

MP01917-1: 85212-2-PBS capture and 85212-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, monkey
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRX2/TXN2 fusion protein Ag3640 相同性解析による交差性が予測される生物種
Full Name thioredoxin 2
Calculated molecular weight 166 aa, 18 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC013726
Gene Symbol Thioredoxin 2
Gene ID (NCBI) 25828
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99757
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TXN2 (Thioredoxin-2) is also named as TRX2, MTRX, Mt-TRX, and regulates the mitochondrial redox state and plays an important role in cell proliferation. It has been shown that cells conditionally deficient in Trx2 undergo apoptosis in the absence of exogenous stress, accompanied by an accumulation of intracellular ROS, the activation of caspase 9 and caspase 3, and the release of cytochrome c into the cytosol. (PMID: 18441302).