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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRNAU1AP,SECP43 Polyclonal antibody (15053-1-AP)

    WB analysis using 15053-1-AP (same clone as 15053-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15053-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TRNAU1AP,SECP43 Polyclonal antibody (15053-1-AP)

    WB analysis of HeLa using 15053-1-AP (same clone as 15053-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15053-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TRNAU1AP,SECP43 Polyclonal antibody (15053-1-AP)

    WB analysis of Jurkat using 15053-1-AP (same clone as 15053-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15053-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using TRNAU1AP,SECP43 Polyclonal antibody (15053-1-AP)

    WB analysis of A549 using 15053-1-AP (same clone as 15053-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15053-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using TRNAU1AP,SECP43 Polyclonal antibody (15053-1-AP)

    WB analysis of COLO 320 using 15053-1-AP (same clone as 15053-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15053-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using TRNAU1AP,SECP43 Polyclonal antibody (15053-1-AP)

    WB analysis of MCF-7 using 15053-1-AP (same clone as 15053-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15053-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using TRNAU1AP,SECP43 Polyclonal antibody (15053-1-AP)

    IHC staining of human hepatocirrhosis using 15053-1-AP (same clone as 15053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15053-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using TRNAU1AP,SECP43 Polyclonal antibody (15053-1-AP)

    IHC staining of human hepatocirrhosis using 15053-1-AP (same clone as 15053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15053-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRNAU1AP,SECP43 Polyclonal antibody, PBS Only

TRNAU1AP,SECP43 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15053-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15053-1-PBS targets TRNAU1AP,SECP43 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7036

Product name: Recombinant human TRNAU1AP,SECP43 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-287 aa of BC000680

Sequence: MAASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHKINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKASRVKPVEYSQMYSYSYNQYYQQYQNYYAQWGYDQNTGSYSYSYPQYGYTQSTMQTYEEVGDDALEDPMPQLDVTEANKEFMEQSEELYDALMDCHWQPLDTVSSEIPAMM

相同性解析による交差性が予測される生物種
Full Name tRNA selenocysteine 1 associated protein 1
Calculated molecular weight 32 kDa
Observed molecular weight 36 kDa, 21 kDa
GenBank accession numberBC000680
Gene Symbol SECP43
Gene ID (NCBI) 54952
RRIDAB_10644291
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX07
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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