Validation Data Gallery

Filter: AllWBIHC

WB analysis using 15053-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 15053-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 15053-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15053-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15053-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15053-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human hepatocirrhosis using 15053-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human hepatocirrhosis using 15053-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15053-1-AP (TRNAU1AP,SECP43 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

TRNAU1AP,SECP43 Polyclonal antibody

TRNAU1AP,SECP43 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15053-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PRO1902, RP4 669K10.4, SECP43, TRNAU1AP, TRNAU1AP,SECP43, TRSPAP1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, Jurkat cells, MCF-7 cells, COLO 320 cells
Positive IHC detected in	human hepatocirrhosis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below

Product Information

15053-1-AP targets TRNAU1AP,SECP43 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRNAU1AP,SECP43 fusion protein Ag7036 相同性解析による交差性が予測される生物種
Full Name	tRNA selenocysteine 1 associated protein 1
Calculated molecular weight	32 kDa
Observed molecular weight	36 kDa, 21 kDa
GenBank accession number	BC000680
Gene Symbol	SECP43
Gene ID (NCBI)	54952
RRID	AB_10644291
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NX07
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TRNAU1AP,SECP43 antibody 15053-1-AP	Download protocol
IHC protocol for TRNAU1AP,SECP43 antibody 15053-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Biochem Biophys Res Commun Knockdown of Trnau1ap inhibits the proliferation and migration of NIH3T3, JEG-3 and Bewo cells via the PI3K/Akt signaling pathway. Authors - Xiaoyan Hu View Article
mouse	WB	Biol Trace Elem Res Selenium Deficiency Leads to Changes in Renal Fibrosis Marker Proteins and Wnt/β-Catenin Signaling Pathway Components. Authors - Tingting Lin View Article
mouse	WB	Biol Trace Elem Res Selenium Deficiency-Induced Damage and Altered Expression of Mitochondrial Biogenesis Markers in the Kidneys of Mice. Authors - Hehuan Lai View Article
rat	WB	Mol Med Rep Role of tRNA selenocysteine 1 associated protein 1 in the proliferation and apoptosis of cardiomyocyte‑like H9c2 cells. Authors - Meng-Di Li KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15053-1-AP

WB analysis of A549 using 15053-1-AP

WB analysis of COLO 320 using 15053-1-AP

WB analysis of HeLa using 15053-1-AP

WB analysis of Jurkat using 15053-1-AP

WB analysis of MCF-7 using 15053-1-AP

IHC staining of human hepatocirrhosis using 15053-1-AP