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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated TRIP6 Monoclonal antibody

TRIP6 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5D8C7

Cat no : CL488-60205

Synonyms

OIP 1, OIP1, Opa interacting protein 1, TR interacting protein 6, TRIP 6, TRIP6, ZRP 1, Zyxin related protein 1

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-60205

    FC experiment of HeLa using CL488-60205

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Trip6 Monoclonal Antibody (CL488-60205, Clone:5D8C7)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HepG2 using CL488-60205

    FC experiment of HepG2 using CL488-60205

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Trip6 Monoclonal Antibody (CL488-60205, Clone:5D8C7)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHepG2 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60205-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-60205 targets TRIP6 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRIP6 fusion protein Ag15149 相同性解析による交差性が予測される生物種
Full Name thyroid hormone receptor interactor 6
Calculated molecular weight 476 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC004249
Gene symbol TRIP6
Gene ID (NCBI) 7205
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIP6 (thyroid receptor-interacting protein 6), also known as ZRP-1 (zyxin-related protein 1), is a member of the zyxin family that has been implicated in actin reorganization and cell motility. Trip6 has been proposed to transport signals from the cell surface to the nucleus.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 TRIP6 antibody CL488-60205Download protocol
Standard Protocols
Click here to view our Standard Protocols