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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using TRIM34 Recombinant antibody (83316-2-RR)

    WB analysis of K-562 using 83316-2-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using TRIM34 Recombinant antibody (83316-2-RR)

    WB analysis of human placenta using 83316-2-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TRIM34 Recombinant antibody (83316-2-RR)

    IF Staining of HEK-293 using 83316-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TRIM34 antibody (83316-2-RR, Clone: 240180E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83316-2-RR

    Affinity and Kinetic Characterization of 83316-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83316-2-RR against Human TRIM34 were performed. The affinity constant is 0.941 nM.

TRIM34 Recombinant monoclonal antibody

TRIM34 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83316-2-RR
Clone No.240180E2

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

IFP1, RING finger protein 21, RNF21, TRIM34, tripartite motif containing 34

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, human placenta tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83316-2-RR targets TRIM34 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34444

Product name: Recombinant human TRIM34 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 34-198 aa of NM_001003827

Sequence: CRACITVSNKEAVTSMGGKSSCPVCGISYSFEHLQANQHLANIVERLKEVKLSPDNGKKRDLCDHHGEKLLLFCKEDRKVICWLCERSQEHRGHHTVLTEEVFKECQEKLQAVLKRLKKEEEEAEKLEADIREEKTSWKYQVQTERQRIQTEFDQLRSILNNEE

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 34
Calculated molecular weight57 kDa
Observed molecular weight31 kDa, 57 kDa
GenBank accession numberNM_001003827
Gene Symbol TRIM34
Gene ID (NCBI) 53840
RRIDAB_3670982
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9BYJ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tripartite motif 34 (TRIM34) is a member of TRIM family that can be highly induced by type I Interferon. TRIM34 has a stimulative role in cell apoptosis and a suppressive role in inflammation. TRIM34 serves a tumor suppressor role in the occurrence and development of colon cancer by enhancing cell apoptosis. TRIM34 possesses at least three kinds of isoforms: the long form at 97 kDa, medium form at 57 kDa, the short form 31 kDa (PMID: 11013086, 31956709).

Protocols

Product Specific Protocols
IF protocol for TRIM34 antibody 83316-2-RRDownload protocol
WB protocol for TRIM34 antibody 83316-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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