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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using TRIM34 Recombinant antibody (83316-2-RR)

    WB analysis of K-562 using 83316-2-RR (same clone as 83316-2-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83316-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using TRIM34 Recombinant antibody (83316-2-RR)

    WB analysis of human placenta using 83316-2-RR (same clone as 83316-2-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83316-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TRIM34 Recombinant antibody (83316-2-RR)

    IF Staining of HEK-293 using 83316-2-RR (same clone as 83316-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TRIM34 antibody (83316-2-RR, Clone: 240180E2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83316-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00340-3

    Cytometric bead array standard curve of MP00340-3

    Cytometric bead array standard curve of MP00340-3, TRIM34 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83316-2-PBS. Detection antibody: 83316-3-PBS. Standard: Ag34444. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 83316-2-RR

    Affinity and Kinetic Characterization of 83316-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83316-2-RR against Human TRIM34 were performed. The affinity constant is 0.941 nM.

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TRIM34 Recombinant antibody, PBS Only (Capture)

TRIM34 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83316-2-PBS
Clone No.240180E2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

240180E2, IFP1, RNF21, tripartite motif containing 34

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83316-2-PBS targets TRIM34 as part of a matched antibody pair:

MP00340-3: 83316-2-PBS capture and 83316-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRIM34 fusion protein Ag34444 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 34
Calculated molecular weight57 kDa
Observed molecular weight31 kDa, 57 kDa
GenBank accession numberNM_001003827
Gene Symbol TRIM34
Gene ID (NCBI) 53840
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BYJ4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Tripartite motif 34 (TRIM34) is a member of TRIM family that can be highly induced by type I Interferon. TRIM34 has a stimulative role in cell apoptosis and a suppressive role in inflammation. TRIM34 serves a tumor suppressor role in the occurrence and development of colon cancer by enhancing cell apoptosis. TRIM34 possesses at least three kinds of isoforms: the long form at 97 kDa, medium form at 57 kDa, the short form 31 kDa (PMID: 11013086, 31956709).