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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TRIM23 Polyclonal antibody (12607-1-AP)

    WB analysis of HEK-293 using 12607-1-AP (same clone as 12607-1-PBS)

    WB result of TRIM23 antibody (12607-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM23 transfected HEK-293 cells. This data was developed using the same antibody clone with 12607-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using TRIM23 Polyclonal antibody (12607-1-AP)

    WB analysis of mouse brain using 12607-1-AP (same clone as 12607-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12607-1-AP (TRIM23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12607-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TRIM23 Polyclonal antibody (12607-1-AP)

    WB analysis of HEK-293 using 12607-1-AP (same clone as 12607-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12607-1-AP (TRIM23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12607-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRIM23 Polyclonal antibody (12607-1-AP)

    IF Staining of HeLa using 12607-1-AP (same clone as 12607-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12607-1-AP (TRIM23 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12607-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRIM23 Polyclonal antibody, PBS Only

TRIM23 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 12607-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12607-1-PBS targets TRIM23 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3302

Product name: Recombinant human TRIM23 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 275-574 aa of BC022510

Sequence: EHVPGTAENARSCIRAYFYDLHETLCRQEEMALSVVDAHVREKLIWLRQQQEDMTILLSEVSAACLHCEKTLQQDDCRVVLAKQEITRLLETLQKQQQQFTEVADHIQLDASIPVTFTKDNRVHIGPKMEIRVVTLGLDGAGKTTILFKLKQDEFMQPIPTIGFNVETVEYKNLKFTIWDVGGKHKLRPLWKHYYLNTQAVVFVVDSSHRDRISEAHSELAKLLTEKELRDALLLIFANKQDVAGALSVEEITELLSLHKLCCGRSWYIQGCDARSGMGLYEGLDWLSRQLVAAGVLDVA

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 23
Calculated molecular weight 574 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC022510
Gene Symbol TRIM23
Gene ID (NCBI) 373
RRIDAB_2256643
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP36406
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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