Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, HEK-293 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
12607-1-AP targets TRIM23 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3302 Product name: Recombinant human TRIM23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 275-574 aa of BC022510 Sequence: EHVPGTAENARSCIRAYFYDLHETLCRQEEMALSVVDAHVREKLIWLRQQQEDMTILLSEVSAACLHCEKTLQQDDCRVVLAKQEITRLLETLQKQQQQFTEVADHIQLDASIPVTFTKDNRVHIGPKMEIRVVTLGLDGAGKTTILFKLKQDEFMQPIPTIGFNVETVEYKNLKFTIWDVGGKHKLRPLWKHYYLNTQAVVFVVDSSHRDRISEAHSELAKLLTEKELRDALLLIFANKQDVAGALSVEEITELLSLHKLCCGRSWYIQGCDARSGMGLYEGLDWLSRQLVAAGVLDVA 相同性解析による交差性が予測される生物種 |
| Full Name | tripartite motif-containing 23 |
| Calculated molecular weight | 574 aa, 64 kDa |
| Observed molecular weight | 64 kDa |
| GenBank accession number | BC022510 |
| Gene Symbol | TRIM23 |
| Gene ID (NCBI) | 373 |
| RRID | AB_2256643 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36406 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM23 antibody 12607-1-AP | Download protocol |
| WB protocol for TRIM23 antibody 12607-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Energy stress promotes P-bodies formation via lysine-63-linked polyubiquitination of HAX1 | ||
Int J Biol Macromol Engineering the orthogonal ubiquitin E1-E2 pairs for identification of K27 chain linkage substrates |
