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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using TRAK2 Polyclonal antibody (13770-1-AP)

    WB analysis of human liver using 13770-1-AP (same clone as 13770-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 13770-1-AP (TRAK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13770-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TRAK2 Polyclonal antibody (13770-1-AP)

    IP experiment of HepG2 using 13770-1-AP (same clone as 13770-1-PBS)

    IP result of anti-TRAK2 (IP:13770-1-AP, 4ug; Detection:13770-1-AP 1:500) with HepG2 cells lysate 4000ug. This data was developed using the same antibody clone with 13770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRAK2 Polyclonal antibody (13770-1-AP)

    IHC staining of human liver cancer using 13770-1-AP (same clone as 13770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13770-1-AP (TRAK2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRAK2 Polyclonal antibody (13770-1-AP)

    IHC staining of human liver cancer using 13770-1-AP (same clone as 13770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13770-1-AP (TRAK2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13770-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAK2 Polyclonal antibody (13770-1-AP)

    IF Staining of HeLa using 13770-1-AP (same clone as 13770-1-PBS)

    Immunofluorescent analysis of Hela cells, using TRAK2 antibody 13770-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13770-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAK2 Polyclonal antibody (13770-1-AP)

    IF Staining of HeLa using 13770-1-AP (same clone as 13770-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAK2 antibody (13770-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13770-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAK2 Polyclonal antibody (13770-1-AP)

    IF Staining of HeLa using 13770-1-AP (same clone as 13770-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13770-1-AP (TRAK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13770-1-PBS in a different storage buffer formulation.

TRAK2 Polyclonal antibody, PBS Only

TRAK2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13770-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Trafficking kinesin-binding protein 2, KIAA0549, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein, ALS2CR3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13770-1-PBS targets TRAK2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4753

Product name: Recombinant human TRAK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-354 aa of BC040668

Sequence: MSQSQNAIFTSPTGEENLMNSNHRDSESITDVCSNEDLPEVELVSLLEEQLPQYRLKVDTLFLYENQDWTQSPHQRQHASDALSPVLAEETFRYMILGTDRVEQMTKTYNDIDMVTHLLAERDRDLELAARIGQALLKRNHILSEQNESLEEQLGQAFDQVNQLQHELCKKDELLRIVSIASEESETDSSCSTPLRFNESFSLSQGLLQLEMLQEKLKELEEENMALRSKACHIKTETVTYEEKEQQLVSDCVKELRETNAQMSRMTEELSGKSDELVRYQEELSSLLSQIVDLQHKLKEHVIEKEELKLHLQASKDAQRQLTMELHELQDRNMECLGMLHESQEEIKELRSRS

相同性解析による交差性が予測される生物種
Full Name trafficking protein, kinesin binding 2
Calculated molecular weight 914 aa, 101 kDa
Observed molecular weight 101-106 kDa
GenBank accession numberBC040668
Gene Symbol TRAK2
Gene ID (NCBI) 66008
RRIDAB_2207961
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60296
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Trafficking kinesin-binding protein 2 (TRAK2) is a member of the TRAK family of kinesin adaptor proteins. In mammals, there are two members of the TRAK family, TRAK1 and TRAK2 (PMID: 25653102). TRAK1 and TRAK2 play roles in lysosomal and mitochondrial trafficking in cells. They function as kinesin adaptors linking kinesin heavy chain (KHC) to mitochondria (PMID: 28364549). TRAK2 has been found to act as a trafficking factor for the K+ channel Kir2.1 and γ-Aminobutyric acid type A (GABAA) receptor (PMID: 16895905; 24122114).

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