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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DcR2 Recombinant antibody (85425-2-RR)

    WB analysis using 85425-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85425-2-RR (DcR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DcR2 Recombinant antibody (85425-2-RR)

    WB analysis using 85425-2-RR

    Human kidney lysate was subjected to SDS PAGE followed by western blot with 85425-2-RR (DcR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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DcR2 Recombinant antibody

DcR2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85425-2-RR
Clone No.242916C11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CD264, Decoy receptor 2, TNF-related apoptosis-inducing ligand receptor 4, TNFRSF10D, TRAIL R4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman kidney tissue, mouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85425-2-RR targets DcR2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DcR2 fusion protein Eg3487 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain
Calculated molecular weight42 kDa
Observed molecular weight50-55 kDa
GenBank accession numberNM_003840.5
Gene Symbol DcR2
Gene ID (NCBI) 8793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBN6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Decoy receptor 2 (DcR2) is a member of the tumor necrosis factor receptor (TNFR) superfamily. It functions as a decoy receptor for TNF-related apoptosis-inducing ligand (TRAIL), thereby inhibiting TRAIL-mediated apoptosis (PMID: 15538968; 9537512). It has been considered a tentative marker of cellular senescence (PMID: 16079833; 22751116). Additionally, DCR2 has been implicated in the regulation of renal fibrosis and cell senescence, contributing to the pathogenesis of diabetic nephropathy (PMID: 35661704).

Protocols

Product Specific Protocols
WB protocol for DcR2 antibody 85425-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols