• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DcR2 Recombinant antibody (85425-2-RR)

    WB analysis using 85425-2-RR (same clone as 85425-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85425-2-RR (DcR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DcR2 Recombinant antibody (85425-2-RR)

    WB analysis using 85425-2-RR (same clone as 85425-2-PBS)

    Human kidney lysate was subjected to SDS PAGE followed by western blot with 85425-2-RR (DcR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01996-1

    Sandwich ELISA standard curve of MP01996-1

    Sandwich ELISA standard curve of MP01996-1, Human DcR2 Recombinant Matched Antibody Pair - PBS only. 85425-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3487. 85425-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

  • Sample test of MP01996-1

    Sample test of MP01996-1

    The mean DcR2 concentration was determined to be 8.72 ng/mL in HCT116 cell extract based on a 1.4 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using DcR2 Recombinant antibody (85425-2-RR)

    IHC staining of mouse liver using 85425-2-RR (same clone as 85425-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85425-2-RR (DcR2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using DcR2 Recombinant antibody (85425-2-RR)

    IHC staining of mouse liver using 85425-2-RR (same clone as 85425-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85425-2-RR (DcR2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01996-1

    Cytometric bead array standard curve of MP01996-1

    Cytometric bead array standard curve of MP01996-1, DcR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85425-1-PBS. Detection antibody: 85425-2-PBS. Standard: Eg3487. Range: 0.781-100 ng/mL.

DcR2 Recombinant monoclonal antibody, PBS Only (Detector)

DcR2 Uni-rAb® Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85425-2-PBS
Clone No.242916C11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD264, Decoy receptor 2, TNF-related apoptosis-inducing ligand receptor 4, TNFRSF10D, TRAIL R4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85425-2-PBS targets DcR2 as part of a matched antibody pair:

MP01996-1: 85425-1-PBS capture and 85425-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3487

Product name: Recombinant Human DcR2 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 56-211 aa of NM_003840.5

Sequence: ATIPRQDEVPQQTVAPQQQRRSLKEEECPAGSHRSEYTGACNPCTEGVDYTIASNNLPSCLLCTVCKSGQTNKSSCTTTRDTVCQCEKGSFQDKNSPEMCRTCRTGCPRGMVKVSNCTPRSDIKCKNESAASSTGKTPAAEETVTTILGMLASPYH

相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain
Calculated molecular weight42 kDa
Observed molecular weight50-55 kDa
GenBank accession numberNM_003840.5
Gene Symbol DcR2
Gene ID (NCBI) 8793
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UBN6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Decoy receptor 2 (DcR2) is a member of the tumor necrosis factor receptor (TNFR) superfamily. It functions as a decoy receptor for TNF-related apoptosis-inducing ligand (TRAIL), thereby inhibiting TRAIL-mediated apoptosis (PMID: 15538968; 9537512). It has been considered a tentative marker of cellular senescence (PMID: 16079833; 22751116). Additionally, DCR2 has been implicated in the regulation of renal fibrosis and cell senescence, contributing to the pathogenesis of diabetic nephropathy (PMID: 35661704).

||
New chat Able
正在加载，请稍候...