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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DcR2 Recombinant antibody (85425-2-RR)

    WB analysis using 85425-2-RR (same clone as 85425-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85425-2-RR (DcR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DcR2 Recombinant antibody (85425-2-RR)

    WB analysis using 85425-2-RR (same clone as 85425-2-PBS)

    Human kidney lysate was subjected to SDS PAGE followed by western blot with 85425-2-RR (DcR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01996-1

    Sandwich ELISA standard curve of MP01996-1

    Sandwich ELISA standard curve of MP01996-1, Human DcR2 Recombinant Matched Antibody Pair - PBS only. 85425-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3487. 85425-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

  • Sample test of MP01996-1

    Sample test of MP01996-1

    The mean DcR2 concentration was determined to be 8.72 ng/mL in HCT116 cell extract based on a 1.4 mg/mL extract load.

  • Cytometric bead array standard curve of MP01996-1

    Cytometric bead array standard curve of MP01996-1

    Cytometric bead array standard curve of MP01996-1, DcR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85425-1-PBS. Detection antibody: 85425-2-PBS. Standard: Eg3487. Range: 0.781-100 ng/mL.

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DcR2 Recombinant antibody, PBS Only (Detector)

DcR2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85425-2-PBS
Clone No.242916C11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD264, Decoy receptor 2, TNF-related apoptosis-inducing ligand receptor 4, TNFRSF10D, TRAIL R4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85425-2-PBS targets DcR2 as part of a matched antibody pair:

MP01996-1: 85425-1-PBS capture and 85425-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DcR2 fusion protein Eg3487 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain
Calculated molecular weight42 kDa
Observed molecular weight50-55 kDa
GenBank accession numberNM_003840.5
Gene Symbol DcR2
Gene ID (NCBI) 8793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBN6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Decoy receptor 2 (DcR2) is a member of the tumor necrosis factor receptor (TNFR) superfamily. It functions as a decoy receptor for TNF-related apoptosis-inducing ligand (TRAIL), thereby inhibiting TRAIL-mediated apoptosis (PMID: 15538968; 9537512). It has been considered a tentative marker of cellular senescence (PMID: 16079833; 22751116). Additionally, DCR2 has been implicated in the regulation of renal fibrosis and cell senescence, contributing to the pathogenesis of diabetic nephropathy (PMID: 35661704).