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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPX2 Recombinant antibody (85118-6-RR)

    WB analysis using 85118-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85118-6-RR (TPX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TPX2 Recombinant antibody (85118-6-RR)

    IHC staining of human cervical cancer using 85118-6-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85118-6-RR (TPX2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TPX2 Recombinant antibody (85118-6-RR)

    IF Staining of HepG2 using 85118-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TPX2 antibody (85118-6-RR, Clone: 242528G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using TPX2 Recombinant antibody (85118-6-RR)

    FC experiment of HeLa using 85118-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TPX2 Recombinant antibody (85118-6-RR, Clone:242528G6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 85118-6-RR

    Affinity and Kinetic Characterization of 85118-6-RR

    Biolayer interferometry (BLl) kinetic assays of 85118-6-RR against Human TPX2 were performed. The affinity constant is 2.24 nM.

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TPX2 Recombinant antibody

TPX2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 85118-6-RR
Clone No.242528G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

242528G6, C20orf1, C20orf2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, SMMC-7721 cells, PANC-1 cells, NIH/3T3 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85118-6-RR targets TPX2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TPX2 fusion protein Ag2334 相同性解析による交差性が予測される生物種
Full Name TPX2, microtubule-associated, homolog (Xenopus laevis)
Calculated molecular weight 747 aa, 86 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC020207
Gene Symbol TPX2
Gene ID (NCBI) 22974
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9ULW0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).

Protocols

Product Specific Protocols
WB protocol for TPX2 antibody 85118-6-RRDownload protocol
IHC protocol for TPX2 antibody 85118-6-RRDownload protocol
IF protocol for TPX2 antibody 85118-6-RRDownload protocol
FC protocol for TPX2 antibody 85118-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols