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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPX2 Recombinant antibody (85118-6-RR)

    WB analysis using 85118-6-RR (same clone as 85118-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85118-6-RR (TPX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85118-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TPX2 Recombinant antibody (85118-6-RR)

    IHC staining of human cervical cancer using 85118-6-RR (same clone as 85118-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85118-6-RR (TPX2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85118-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TPX2 Recombinant antibody (85118-6-RR)

    IF Staining of HepG2 using 85118-6-RR (same clone as 85118-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TPX2 antibody (85118-6-RR, Clone: 242528G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85118-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TPX2 Recombinant antibody (85118-6-RR)

    FC experiment of HeLa using 85118-6-RR (same clone as 85118-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TPX2 Recombinant antibody (85118-6-RR, Clone:242528G6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85118-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85118-6-RR

    Affinity and Kinetic Characterization of 85118-6-RR

    Biolayer interferometry (BLl) kinetic assays of 85118-6-RR against Human TPX2 were performed. The affinity constant is 2.24 nM.

TPX2 Recombinant monoclonal antibody, PBS Only

TPX2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85118-6-PBS
Clone No.242528G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242528G6, C20orf1, C20orf2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85118-6-PBS targets TPX2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2334

Product name: Recombinant human TPX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-395 aa of BC020207

Sequence: MSQVKSSYSYDAPSDFINFSSLDDEGDTQNIDSWFEEKANLENKLLGKNGTGGLFQGKTPLRKANLQQAIVTPLKPVDNTYYKEAEKENLVEQSIPSNACSSLEVEAAISRKTPAQPQRRSLRLSAQKDLEQKEKHHVKMKAKRCATPVIIDEILPSKKMKVSNNKKKPEEEGSAHQDTAEKNASSPEKAKGRHTVPCMPPAKQKFLKSTEEQELEKSMKMQQEVVEMRKKNEEFKKLALAGIGQPVKKSVSQVTKSVDFHFRTDERIKQHPKNQEEYKEVNFTSELRKHPSSPARVTKGCTIVKPFNLSQGKKRTFDETVSTYVPLAQQVEDFHKRTPNRYHLRSKKDDINLLPSKSSVTKICRDPQTPVLQTKHRARAVTCKSTAELEAEELE

相同性解析による交差性が予測される生物種
Full Name TPX2, microtubule-associated, homolog (Xenopus laevis)
Calculated molecular weight 747 aa, 86 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC020207
Gene Symbol TPX2
Gene ID (NCBI) 22974
RRIDAB_3744026
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9ULW0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).

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