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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPM4 Monoclonal antibody (67244-1-Ig)

    WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TPM4 Monoclonal antibody (67244-1-Ig)

    WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TPM4 Monoclonal antibody (67244-1-Ig)

    WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using TPM4 Monoclonal antibody (67244-1-Ig)

    WB analysis of A549 using 67244-1-Ig (same clone as 67244-1-PBS)

    WB result of TPM4 antibody (67244-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM4 transfected A549 cells. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TPM4 Monoclonal antibody (67244-1-Ig)

    IHC staining of mouse heart using 67244-1-Ig (same clone as 67244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using TPM4 Monoclonal antibody (67244-1-Ig)

    IHC staining of rat heart using 67244-1-Ig (same clone as 67244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TPM4 Monoclonal antibody (67244-1-Ig)

    IF Staining of MCF-7 using 67244-1-Ig (same clone as 67244-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TPM4 antibody (67244-1-Ig, Clone: 2E12D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TPM4 Monoclonal antibody, PBS Only

TPM4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67244-1-PBS
Clone No.2E12D12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

2E12D12, TM30p1, tropomyosin 4, Tropomyosin alpha 4 chain, Tropomyosin alpha-4 chain

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67244-1-PBS targets TPM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6947

Product name: Recombinant human TPM4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-248 aa of BC037576

Sequence: MAGLNSLEAVKRKIQALQQQADEAEDRAQGLQRELDGERERREKAEGDVAALNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEKMEIQEMQLKEAKHIAEEADRKYEEVARKLVILEGELERAEERAEVSELKCGDLEEELKNVTNNLKSLEAASEKYSEKEDKYEEEIKLLSDKLKEAETRAEFAERTVAKLEKTIDDLEEKLAQAKEENVGLHQTLDQTLNELNCI

相同性解析による交差性が予測される生物種
Full Name tropomyosin 4
Calculated molecular weight 248 aa, 29 kDa
Observed molecular weight 32-35 kDa
GenBank accession numberBC037576
Gene Symbol TPM4
Gene ID (NCBI) 7171
ENSEMBL Gene IDENSG00000167460
RRIDAB_2882523
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP67936
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TPM4 is a member of tropomyosins (TPMs), a multi-gene family of actin-binding proteins present in all eukaryotic cells. In muscle, TPMs are responsible for mediating contraction via regulation of the actin-myosin interaction. As to non-muscle cells, its proposed role is to stabilize the actin filaments by modulating the interaction with proteins that are responsible for the regulation of actin dynamics. The form of TPM4 in muscle has a higher molecular weight than the form found in non-muscle cells.