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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TP53BP1-Specific Polyclonal antibody (20002-1-AP)

    WB analysis using 20002-1-AP (same clone as 20002-1-PBS)

    HeLa lysates were subjected to SDS PAGE followed by western blot with 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TP53BP1-Specific Polyclonal antibody (20002-1-AP)

    WB analysis using 20002-1-AP (same clone as 20002-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TP53BP1-Specific Polyclonal antibody (20002-1-AP)

    IHC staining of human stomach cancer using 20002-1-AP (same clone as 20002-1-PBS)

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TP53BP1-Specific Polyclonal antibody (20002-1-AP)

    IHC staining of human stomach cancer using 20002-1-AP (same clone as 20002-1-PBS)

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TP53BP1-Specific Polyclonal antibody (20002-1-AP)

    IF Staining of HeLa using 20002-1-AP (same clone as 20002-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TP53BP1-Specific antibody (20002-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

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TP53BP1-Specific Polyclonal antibody, PBS Only

TP53BP1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20002-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

TP53BP1, 53BP1, FLJ41424, p202, p53 binding protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20002-1-PBS targets TP53BP1-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tumor protein p53 binding protein 1
Calculated molecular weight 214 kDa
Observed molecular weight450 kDa
GenBank accession numberNM_005657
Gene Symbol TP53BP1
Gene ID (NCBI) 7158
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12888
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TP53BP1, also named as p53BP1, p202, 53BP1 and FLJ41424, may have a role in checkpoint signaling during mitosis. TP53BP1 enhances TP53-mediated transcriptional activation. TP53BP1 plays a role in the response to DNA damage. This antibody is specific to TP53BP1.