Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, C6 cells, NIH/3T3 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
20002-1-AP targets TP53BP1-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | tumor protein p53 binding protein 1 |
| Calculated molecular weight | 214 kDa |
| Observed molecular weight | 450 kDa |
| GenBank accession number | NM_005657 |
| Gene Symbol | TP53BP1 |
| Gene ID (NCBI) | 7158 |
| RRID | AB_3085592 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12888 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TP53BP1, also named as p53BP1, p202, 53BP1 and FLJ41424, may have a role in checkpoint signaling during mitosis. TP53BP1 enhances TP53-mediated transcriptional activation. TP53BP1 plays a role in the response to DNA damage. This antibody is specific to TP53BP1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TP53BP1-Specific antibody 20002-1-AP | Download protocol |
| IHC protocol for TP53BP1-Specific antibody 20002-1-AP | Download protocol |
| WB protocol for TP53BP1-Specific antibody 20002-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Heliyon Cyclin-dependent kinase inhibitor 1A inhibits pyroptosis to enhance human lung adenocarcinoma cell radioresistance by promoting DNA repair | ||
Stem Cells Transl Med Mesenchymal stromal cells-derived small extracellular vesicles protect against UV-induced photoaging via regulating pregnancy zone protein | ||
Oncogene Phospho-TRIM21 orchestrates RPA2 ubiquitination switch to promote homologous recombination and tumor radio/chemo-resistance |