×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • SEC-HPLC of 82738-10-RR

    SEC-HPLC of 82738-10-RR

    The purity of Human TOP2A-Specific Recombinant monoclonal antibody (Cat. No. 82738-10-RR) was greater than 95% as determined by SEC-HPLC.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TOP2A-Specific Recombinant monoclonal antibody (82738-10-RR)

    IHC staining of human tonsillitis using 82738-10-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82738-10-RR (TOP2A-Specific antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TOP2A-Specific Recombinant monoclonal antibody (82738-10-RR)

    IHC staining of human tonsillitis using 82738-10-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82738-10-RR (TOP2A-Specific antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TOP2A-Specific Recombinant monoclonal antibody (82738-10-RR)

    IF Staining of U2OS using 82738-10-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TOP2A-Specific antibody (82738-10-RR, Clone: 253104A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

TOP2A-Specific Recombinant monoclonal antibody

TOP2A-Specific Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 82738-10-RR
Clone No.253104A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

TOP2A, DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, EC:5.6.2.2, TOP II

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82738-10-RR targets TOP2A-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name topoisomerase (DNA) II alpha 170kDa
Calculated molecular weight 174 kDa
GenBank accession numberNM_001067
Gene Symbol TOP2A
Gene ID (NCBI) 7153
RRIDAB_3743420
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP11388
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Protocols

Product Specific Protocols
IF protocol for TOP2A-Specific antibody 82738-10-RRDownload protocol
IHC protocol for TOP2A-Specific antibody 82738-10-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...