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Validation Data Gallery

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  • SEC-HPLC of 82738-10-RR

    SEC-HPLC of 82738-10-RR

    The purity of Human TOP2A-Specific Recombinant monoclonal antibody (Cat. No. 82738-10-RR) was greater than 95% as determined by SEC-HPLC.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TOP2A-Specific Recombinant monoclonal antibody (82738-10-RR)

    IHC staining of human tonsillitis using 82738-10-RR (same clone as 82738-10-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82738-10-RR (TOP2A-Specific antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82738-10-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TOP2A-Specific Recombinant monoclonal antibody (82738-10-RR)

    IHC staining of human tonsillitis using 82738-10-RR (same clone as 82738-10-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82738-10-RR (TOP2A-Specific antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82738-10-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TOP2A-Specific Recombinant monoclonal antibody (82738-10-RR)

    IF Staining of U2OS using 82738-10-RR (same clone as 82738-10-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TOP2A-Specific antibody (82738-10-RR, Clone: 253104A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82738-10-PBS in a different storage buffer formulation.

TOP2A-Specific Recombinant monoclonal antibody, PBS Only

TOP2A-Specific Uni-rAb® Recombinant Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 82738-10-PBS
Clone No.253104A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

TOP2A, DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, EC:5.6.2.2, TOP II

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82738-10-PBS targets TOP2A-Specific in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name topoisomerase (DNA) II alpha 170kDa
Calculated molecular weight 174 kDa
GenBank accession numberNM_001067
Gene Symbol TOP2A
Gene ID (NCBI) 7153
RRIDAB_3743420
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP11388
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

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