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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOP2A Recombinant antibody (82738-4-RR)

    WB analysis using 82738-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82738-4-RR (TOP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using TOP2A Recombinant antibody (82738-4-RR)

    IP experiment of HeLa using 82738-4-RR

    IP result of anti-TOP2A (IP:82738-4-RR, 4ug; Detection:82738-4-RR 1:2000) with HeLa cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TOP2A Recombinant monoclonal antibody (82738-4-RR)

    IHC staining of human tonsillitis using 82738-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82738-4-RR (TOP2A antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TOP2A Recombinant antibody (82738-4-RR)

    IF Staining of A431 using 82738-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOP2A antibody (82738-4-RR, Clone: 1J18 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82738-4-RR

    Affinity and Kinetic Characterization of 82738-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82738-4-RR against Human TOP2A were performed. The affinity constant is 0.928 nM.

TOP2A Recombinant monoclonal antibody

TOP2A Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 82738-4-RR
Clone No.1J18

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

1J18, DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, EC:5.6.2.2, TOP II

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HeLa cells, HEK-293 cells, A431 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82738-4-RR targets TOP2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19551

Product name: Recombinant human TOP2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1187-1531 aa of BC140791

Sequence: QDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF

相同性解析による交差性が予測される生物種
Full Name topoisomerase (DNA) II alpha 170kDa
Calculated molecular weight 1531 aa, 174 kDa
Observed molecular weight~170 kDa
GenBank accession numberBC140791
Gene Symbol TOP2A
Gene ID (NCBI) 7153
RRIDAB_3086521
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP11388
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Protocols

Product Specific Protocols
IF protocol for TOP2A antibody 82738-4-RRDownload protocol
IHC protocol for TOP2A antibody 82738-4-RRDownload protocol
IP protocol for TOP2A antibody 82738-4-RRDownload protocol
WB protocol for TOP2A antibody 82738-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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