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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOP2A Recombinant monoclonal antibody (82738-4-RR)

    WB analysis using 82738-4-RR (same clone as 82738-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82738-4-RR (TOP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82738-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using TOP2A Recombinant monoclonal antibody (82738-4-RR)

    IP experiment of HeLa using 82738-4-RR (same clone as 82738-4-PBS)

    IP result of anti-TOP2A (IP:82738-4-RR, 4ug; Detection:82738-4-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82738-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TOP2A Recombinant monoclonal antibody (82738-4-RR)

    IHC staining of human tonsillitis using 82738-4-RR (same clone as 82738-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82738-4-RR (TOP2A antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82738-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TOP2A Recombinant monoclonal antibody (82738-4-RR)

    IF Staining of A431 using 82738-4-RR (same clone as 82738-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOP2A antibody (82738-4-RR, Clone: 1J18 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red). This data was developed using the same antibody clone with 82738-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82738-4-RR

    Affinity and Kinetic Characterization of 82738-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82738-4-RR against Human TOP2A were performed. The affinity constant is 0.928 nM.

TOP2A Recombinant monoclonal antibody, PBS Only

TOP2A Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82738-4-PBS
Clone No.1J18

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

1J18, DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, EC:5.6.2.2, TOP II

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82738-4-PBS targets TOP2A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19551

Product name: Recombinant human TOP2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1187-1531 aa of BC140791

Sequence: QDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF

相同性解析による交差性が予測される生物種
Full Name topoisomerase (DNA) II alpha 170kDa
Calculated molecular weight 1531 aa, 174 kDa
Observed molecular weight~170 kDa
GenBank accession numberBC140791
Gene Symbol TOP2A
Gene ID (NCBI) 7153
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP11388
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

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