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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated TOE1 Monoclonal antibody

TOE1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C10H7

Cat no : CL488-67829

Synonyms

FLJ13949, Target of EGR1 protein 1, TOE1

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-67829

    IF Staining of HepG2 using CL488-67829

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TOE1 antibody (CL488-67829, Clone: 2C10H7 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-67829

    FC experiment of HepG2 using CL488-67829

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TOE1 (CL488-67829, Clone:2C10H7) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67829-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67829 targets TOE1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TOE1 fusion protein Ag28982 相同性解析による交差性が予測される生物種
Full Name target of EGR1, member 1 (nuclear)
Calculated molecular weight 510 aa, 57 kDa
Observed molecular weight57 kDa
GenBank accession numberBC009364
Gene symbol TOE1
Gene ID (NCBI) 114034
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TOE1 antibody CL488-67829Download protocol
FC protocol for CL Plus 488 TOE1 antibody CL488-67829Download protocol
Standard Protocols
Click here to view our Standard Protocols