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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNRC18 Polyclonal antibody (32165-1-AP)

    WB analysis using 32165-1-AP (same clone as 32165-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32165-1-AP (TNRC18 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32165-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TNRC18 Polyclonal antibody (32165-1-AP)

    WB analysis using 32165-1-AP (same clone as 32165-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32165-1-AP (TNRC18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32165-1-PBS in a different storage buffer formulation.

TNRC18 Polyclonal antibody, PBS Only

TNRC18 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 32165-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Trinucleotide repeat-containing gene 18 protein, Trinucleotide Repeat Containing 18, Long CAG trinucleotide repeat-containing gene 79 protein, KIAA1856, CAGL79

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

32165-1-PBS targets TNRC18 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag37483

Product name: Recombinant human TNRC18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1238-1400 aa of BC131808

Sequence: PCTAALDLGVQLTPETLVEAKEEPVEVPVAVPVVEAVPEEGLAQVAPSESQPTLEMSDCDVPAGEGQCPSLEPQEAVPVLGSTCFLEEASSDQFLPSLEDPLAGMNALAAAAELPQARPLPSPGAAGAQALEKLEAAESLVLEQSFLHGITLLSEIAELELER

相同性解析による交差性が予測される生物種
Full Name trinucleotide repeat containing 18
Calculated molecular weight31 kDa
Observed molecular weight29 kDa
GenBank accession numberBC131808
Gene Symbol TNRC18
Gene ID (NCBI) 84629
RRIDAB_3670212
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDO15417
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Trinucleotide repeat containing 18 (TNRC18, also known as CAGL79 and KIAA1856) is a nucleic-acid-binding protein, which recognizes H3K9me3 to mediate the silencing of ERV class I (ERV1) elements and acts as a chromatin-associated regulator of ERVs (PMID: 27980689; 37938770). It is located in the cytosol, mitochondrion, and nucleus (PMID: 19262598). The mutation in TNRC18 may cause Fazio-Londe disease (PMID: 38188848).

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