Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, HepG2 cells, MDA-MB-453 cells, mouse kidney tissue, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32165-1-AP targets TNRC18 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37483 Product name: Recombinant human TNRC18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1238-1400 aa of BC131808 Sequence: PCTAALDLGVQLTPETLVEAKEEPVEVPVAVPVVEAVPEEGLAQVAPSESQPTLEMSDCDVPAGEGQCPSLEPQEAVPVLGSTCFLEEASSDQFLPSLEDPLAGMNALAAAAELPQARPLPSPGAAGAQALEKLEAAESLVLEQSFLHGITLLSEIAELELER 相同性解析による交差性が予測される生物種 |
| Full Name | trinucleotide repeat containing 18 |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC131808 |
| Gene Symbol | TNRC18 |
| Gene ID (NCBI) | 84629 |
| RRID | AB_3670212 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O15417 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Trinucleotide repeat containing 18 (TNRC18, also known as CAGL79 and KIAA1856) is a nucleic-acid-binding protein, which recognizes H3K9me3 to mediate the silencing of ERV class I (ERV1) elements and acts as a chromatin-associated regulator of ERVs (PMID: 27980689; 37938770). It is located in the cytosol, mitochondrion, and nucleus (PMID: 19262598). The mutation in TNRC18 may cause Fazio-Londe disease (PMID: 38188848).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TNRC18 antibody 32165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |