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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFSF15 Polyclonal antibody (29899-1-AP)

    WB analysis using 29899-1-AP (same clone as 29899-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29899-1-AP (TNFSF15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29899-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TNFSF15 Polyclonal antibody (29899-1-AP)

    WB analysis using 29899-1-AP (same clone as 29899-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29899-1-AP (TNFSF15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29899-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TNFSF15 Polyclonal antibody (29899-1-AP)

    WB analysis using 29899-1-AP (same clone as 29899-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 29899-1-AP (TNFSF15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29899-1-PBS in a different storage buffer formulation.

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TNFSF15 Polyclonal antibody, PBS Only

TNFSF15 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 29899-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29899-1-PBS targets TNFSF15 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 15
Calculated molecular weight 28 kDa
Observed molecular weight28 kDa
GenBank accession numberBC074941
Gene Symbol TNFSF15
Gene ID (NCBI) 9966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95150
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Tumor necrosis factor ligand superfamily member 15 (TNFSF15) is also named as TL1 and VEGI. And it belongs to the tumor necrosis factor family. It is a receptor for TNFRSF25 and TNFRSF6B. VEGI induced degradation of IκB alpha, and nuclear translocation of p65 subunit of NFκB by activating NFκB (PMID: 10597252). TL1A mediates activation and apoptosis of NFκB in DR3-expressing cell lines (PMID: 11911831). Overexpression by cancer cells of a secretable VEGI fusion protein resulted in abrogation of xenograft tumor progression. The isoforms show endothelial cell-specific expression and are generated from a 17 kb human gene by alternative splicing (PMID: 11923219). In addition, VEGI can inhibit vascular endothelial growth and angiogenesis (in vitro) (PMID: 9872942).