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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFSF15 Polyclonal antibody (29899-1-AP)

    WB analysis using 29899-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29899-1-AP (TNFSF15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TNFSF15 Polyclonal antibody (29899-1-AP)

    WB analysis using 29899-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29899-1-AP (TNFSF15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TNFSF15 Polyclonal antibody (29899-1-AP)

    WB analysis using 29899-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 29899-1-AP (TNFSF15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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TNFSF15 Polyclonal antibody

TNFSF15 Polyclonal Antibody for WB, ELISA
Cat No. 29899-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Tumor necrosis factor ligand superfamily member 15, secreted form, Tumor necrosis factor ligand superfamily member 15, membrane form, Tumor necrosis factor ligand superfamily member 15, TNFSF 15

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse colon tissue, mouse small intestine tissue, rat small intestine tissue, rat colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29899-1-AP targets TNFSF15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 15
Calculated molecular weight 28 kDa
Observed molecular weight28 kDa
GenBank accession numberBC074941
Gene Symbol TNFSF15
Gene ID (NCBI) 9966
RRIDAB_3086184
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95150
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tumor necrosis factor ligand superfamily member 15 (TNFSF15) is also named as TL1 and VEGI. And it belongs to the tumor necrosis factor family. It is a receptor for TNFRSF25 and TNFRSF6B. VEGI induced degradation of IκB alpha, and nuclear translocation of p65 subunit of NFκB by activating NFκB (PMID: 10597252). TL1A mediates activation and apoptosis of NFκB in DR3-expressing cell lines (PMID: 11911831). Overexpression by cancer cells of a secretable VEGI fusion protein resulted in abrogation of xenograft tumor progression. The isoforms show endothelial cell-specific expression and are generated from a 17 kb human gene by alternative splicing (PMID: 11923219). In addition, VEGI can inhibit vascular endothelial growth and angiogenesis (in vitro) (PMID: 9872942).

Protocols

Product Specific Protocols
WB protocol for TNFSF15 antibody 29899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

J Orthop Translat

Targeting the senescence-related genes MAPK12 and FOS to alleviate osteoarthritis

Authors - Nana Geng
View Article