Validation Data Gallery
Tested Applications
| Positive FC detected in | human peripheral blood leukocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-FcA98085 targets TNFR1/CD120a in FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant Protein 相同性解析による交差性が予測される生物種 |
| Full Name | tumor necrosis factor receptor superfamily, member 1A |
| Calculated molecular weight | 455 aa, 50 kDa |
| GenBank accession number | BC010140 |
| Gene Symbol | TNFR1 |
| Gene ID (NCBI) | 7132 |
| RRID | AB_3746905 |
| Conjugate | Biotin |
| Excitation/Emission maxima wavelengths | - |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P19438 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Biotin TNFR1/CD120a antibody Biotin-FcA98085 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |